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Bilirubin (Direct/Indirect)

Organ Function & Health
65.00

Private Bilirubin (Direct/Indirect) blood test in London for £65, measuring three bilirubin markers with results in around 1 business day.

Turnaround time

1 day

Biomarkers count

1

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Under 18? Patients under 18 can only be seen at GB Medlabs and Clinilabs, Monday to Friday only.
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Overview of the Bilirubin (Direct/Indirect)

The Bilirubin (Direct/Indirect) blood test provides a detailed assessment of bilirubin by measuring total bilirubin together with its direct and indirect components.

Bilirubin is a yellow pigment produced mainly during the normal breakdown of haemoglobin from ageing red blood cells.

The liver processes bilirubin so that it can be excreted through bile and ultimately removed from the body.

The test contains 3 biomarkers: Bilirubin (Total), Direct Bilirubin and Indirect Bilirubin.

Indirect bilirubin, also called unconjugated bilirubin, is the form circulating before it has been processed by the liver.

Direct bilirubin, also called conjugated bilirubin, has been processed by liver cells so that it can be excreted into bile.

Separating an elevated total bilirubin into direct and indirect fractions can provide useful information about where the abnormality may be occurring.

Predominantly raised indirect bilirubin can occur with increased red blood cell breakdown or conditions affecting bilirubin conjugation, including Gilbert's syndrome.

Predominantly raised direct bilirubin can occur when bilirubin has been conjugated but cannot be excreted normally, including some liver and bile duct conditions.

The pattern should be interpreted alongside liver enzymes, Full Blood Count, symptoms and other investigations rather than used independently to diagnose a liver, gallbladder or haematological disorder.

What Does the Bilirubin (Direct/Indirect) Test Check?

This test measures 3 biomarkers.

Bilirubin Metabolism
Bilirubin (Total)

Measures the total amount of bilirubin circulating in the blood, combining the direct and indirect fractions.

Direct Bilirubin

Measures conjugated bilirubin that has already been processed by the liver. Increased concentrations may occur when bilirubin excretion or bile flow is impaired.

Indirect Bilirubin

Measures unconjugated bilirubin before liver conjugation. Increased concentrations can occur with increased red blood cell breakdown or reduced bilirubin conjugation.

Who May Benefit From the Bilirubin (Direct/Indirect) Test?

This test may be suitable when total bilirubin is abnormal or when a clinician wants to determine whether an elevation is predominantly direct or indirect.

People with unexplained jaundice - Separating bilirubin into fractions can provide useful information when the skin or eyes appear yellow.

People with a previously raised total bilirubin - Fractionation can help establish whether the abnormality predominantly involves direct or indirect bilirubin.

Possible Gilbert's syndrome - Isolated mild unconjugated bilirubin elevation can occur in Gilbert's syndrome.

Possible increased red blood cell breakdown - Haemolysis can increase indirect bilirubin and usually requires assessment alongside other haematology markers.

Possible liver disease - Direct bilirubin may increase when liver cells cannot excrete conjugated bilirubin normally.

Possible bile duct obstruction - Cholestasis or biliary obstruction can produce a predominantly conjugated bilirubin pattern.

People with abnormal liver-function results - Bilirubin fractionation can provide additional information alongside ALT, AST, ALP and GGT.

People being monitored for an established liver condition - Repeat bilirubin measurements may help track changes over time.

Specialist-requested bilirubin fractionation - The test can be arranged privately where a GP, hepatologist, gastroenterologist or haematologist has requested direct and indirect bilirubin.

How to Prepare

Fasting
Fasting is not normally required unless another test being performed at the same appointment requires it. Prolonged fasting can increase bilirubin in some people, particularly those with Gilbert's syndrome.
Hydration
Drink water normally before your appointment. Dehydration can sometimes contribute to temporary bilirubin variation.
Medication
Continue prescribed medication unless your GP or specialist specifically advises otherwise. Some medicines can influence bilirubin metabolism or bile flow.
Alcohol
Avoid excessive alcohol intake before testing, particularly where liver health is being investigated.
Recent illness
Tell the clinician about recent illness, infection or significant physical stress because these can influence bilirubin concentrations.
Previous results
Keep previous total bilirubin, direct bilirubin, liver-function or haematology results where available.
Timing
The test can generally be performed at any time of day.

Do not deliberately fast for prolonged periods simply to reproduce a previous bilirubin elevation.

Bilirubin results are most useful when interpreted alongside other liver and haematology markers and in the context of any symptoms.

Symptoms and Reasons to Consider the Bilirubin (Direct/Indirect) Test

You may wish to consider the Bilirubin (Direct/Indirect) test where jaundice or previous blood results suggest abnormal bilirubin metabolism.

Yellowing of the skin or eyes - Jaundice occurs when bilirubin accumulates sufficiently to become visible in tissues.

Dark urine - Conjugated bilirubin can enter the urine when blood levels are increased.

Pale stools - Reduced bile reaching the intestine can cause stools to become unusually pale.

Unexplained fatigue - Fatigue can occur with liver disease or haemolysis but is highly non-specific.

Upper abdominal discomfort - Liver, gallbladder and biliary conditions can occasionally cause abdominal symptoms.

Intermittent mild jaundice during illness or fasting - Gilbert's syndrome commonly causes fluctuating unconjugated bilirubin elevations during physiological stress.

Previous high total bilirubin - Fractionation can help clarify whether the elevation is direct or indirect.

Abnormal liver enzymes - Bilirubin fractions may provide additional context when other liver-related markers are abnormal.

Jaundice has many possible causes, ranging from benign inherited bilirubin metabolism to liver disease, bile duct obstruction and increased red blood cell breakdown.

The bilirubin fractions help identify biochemical patterns but do not determine the diagnosis by themselves.

New jaundice, severe abdominal pain, fever with jaundice, persistent vomiting, confusion or rapidly worsening illness requires prompt medical assessment.

How to Book Your Bilirubin (Direct/Indirect) Test

Booking your Bilirubin (Direct/Indirect) test with London Blood Tests is simple and designed to fit around your schedule.

1
Choose the Bilirubin (Direct/Indirect)

Select the Bilirubin (Direct/Indirect) test online and review the three included bilirubin biomarkers before booking.

2
Select your sample collection option

Choose an in-clinic blood draw (+£35) or a home or hotel phlebotomy visit (+£60) where a qualified phlebotomist collects your sample.

3
Attend your appointment

A trained professional will collect the required venous blood sample.

4
Laboratory analysis

Your sample is sent to the laboratory, where total, direct and indirect bilirubin are measured.

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Receive your results securely

Your results are sent securely by email, ready to review yourself or discuss with a GP, hepatologist, gastroenterologist or other suitably qualified healthcare professional.

When Will I Receive My Results?

The expected turnaround time for the Bilirubin (Direct/Indirect) test is approximately 1 business day after your sample arrives at the laboratory.

Results are sent securely once laboratory analysis and authorisation are complete.

Additional checks or repeat analysis may occasionally extend the expected turnaround.

Understanding Your Results

Your report shows total bilirubin together with direct and indirect bilirubin concentrations.

If total bilirubin is raised, the relative proportions of direct and indirect bilirubin can help guide further investigation.

Predominantly raised indirect bilirubin may occur when bilirubin production is increased or liver conjugation is reduced.

Common examples include Gilbert's syndrome and haemolysis, although additional tests are required to distinguish between causes.

Predominantly raised direct bilirubin can occur with impaired bile excretion, cholestasis, biliary obstruction or hepatocellular disease.

A mixed elevation can occur where several processes affect bilirubin production and excretion simultaneously.

Normal bilirubin fractions do not exclude every liver or gallbladder disorder.

Results should be interpreted alongside ALT, AST, ALP, GGT, Full Blood Count, haptoglobin and other investigations where appropriate.

Reference intervals vary between laboratories, so interpretation should follow the ranges stated on your report.

Why Book With London Blood Tests?

Private bilirubin fractionation in London - Access total, direct and indirect bilirubin testing through London Blood Tests.

Three related bilirubin measurements - Assess the bilirubin pattern rather than relying on total bilirubin alone.

Useful for jaundice investigation - Direct and indirect fractions can provide additional information about bilirubin production and excretion.

Relevant to Gilbert's syndrome assessment - Predominantly unconjugated bilirubin elevation may provide supporting information.

Useful alongside liver-function testing - Bilirubin fractions can complement liver enzymes and bile-flow markers.

Fast laboratory turnaround - Results are expected approximately 1 business day after the laboratory receives your sample.

Clinic and home visit options - Choose an in-clinic blood draw or arrange home or hotel phlebotomy depending on availability.

Secure results by email - Receive your authorised laboratory report securely.

Clear pricing before booking - The Bilirubin (Direct/Indirect) test is priced at £65, with sample collection charges shown separately.

Private Bilirubin (Direct/Indirect) Blood Test in London

London Blood Tests provides the Bilirubin (Direct/Indirect) blood test in London for people requiring detailed assessment of bilirubin metabolism.

The test costs £65 and measures three biomarkers: Bilirubin (Total), Direct Bilirubin and Indirect Bilirubin.

Testing may be useful when jaundice is present, total bilirubin has previously been elevated or a clinician wants to distinguish conjugated from unconjugated hyperbilirubinaemia.

Clinic and home or hotel collection options are available depending on location and availability.

Results should be assessed alongside symptoms and other liver or haematology investigations rather than used independently.

Frequently Asked Questions

It measures total bilirubin, direct bilirubin and indirect bilirubin.

The test contains 3 biomarkers: Bilirubin (Total), Direct Bilirubin and Indirect Bilirubin.

The Bilirubin (Direct/Indirect) test costs £65. Sample collection charges apply separately.

Direct bilirubin is bilirubin that has been conjugated and processed by the liver.

Indirect bilirubin is unconjugated bilirubin circulating before liver processing.

Causes include Gilbert's syndrome and increased red blood cell breakdown, among others.

Direct bilirubin can increase with cholestasis, bile duct obstruction and some liver conditions.

Yes. Prolonged fasting can increase bilirubin, particularly in people with Gilbert's syndrome.

No. Bilirubin can be elevated for several hepatic, biliary, haematological and inherited reasons.

Fasting is not normally required unless another test requires it.

Results are expected approximately 1 business day after the sample reaches the laboratory.
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