Understanding the Gluten Allergy Profile blood test

The Gluten Allergy Profile is an advanced diagnostic blood test that evaluates multiple immune pathways related to gluten reactions. It is designed to distinguish between IgE-mediated gluten allergy, coeliac disease, and non-allergic immune responses, while also assessing genetic predisposition.

Rather than relying on a single marker, this profile combines allergy antibodies, autoimmune antibodies, genetic testing, and immune status markers to provide a clear, clinically meaningful interpretation of gluten-related symptoms, especially in cases where symptoms are complex or long-standing.

The Gluten Allergy Profile includes:

• Gluten single IgE Allergen
• Endomysial Antibodies IgA
• Deamidated Gliadin IgG Antibodies
• Tissue Transglutaminase IgA
• Coeliac Disease – HLA DQ2/DQ8 Genotype
• Total IgA

Together, these markers allow accurate differentiation between true gluten allergy, coeliac disease, genetic susceptibility, and alternative causes of symptoms.

When a Gluten Allergy Profile blood test is recommended

This test is recommended when gluten-related symptoms are present but the underlying cause is unclear or conflicting. Many individuals eliminate gluten without a definitive diagnosis, which can delay appropriate treatment or monitoring.

The Gluten Allergy Profile is particularly useful when symptoms persist despite dietary changes or when previous testing has been incomplete.

Testing may be appropriate if you:

• Experience digestive symptoms after eating gluten
• Have bloating, diarrhoea, or abdominal pain
• Have unexplained fatigue or brain fog
• Have iron deficiency or anaemia of unclear cause
• Have skin rashes such as dermatitis herpetiformis
• Have a family history of coeliac disease
• Have suspected food allergy reactions
• Want a definitive assessment before dietary restriction

Benefits of the Gluten Allergy Profile

• Differentiates allergy from coeliac disease
  Separates IgE-mediated allergy from autoimmune disease.
• Includes genetic coeliac risk assessment
  HLA DQ2/DQ8 testing clarifies lifelong risk.
• Assesses immune response accuracy
  Total IgA ensures coeliac antibodies are reliable.
• Identifies silent or atypical coeliac disease
  Detects non-classical presentations.
• Reduces diagnostic uncertainty
  Combines all key markers in one profile.
• Prevents unnecessary long-term dietary restriction
  Supports evidence-based decisions.
• Simple blood test with no invasive procedures
  No biopsies required at screening stage.
• Provides clear clinical direction
  Guides referral, monitoring, or reassurance.

How the test is carried out

We offer two convenient UK-wide testing options:

Clinic-based blood testing

• Choose your nearest UK clinic
• A healthcare professional collects a blood sample
• The sample is analysed in certified specialist laboratories
• Results are issued once testing is complete

Home or hotel blood collection (+£60)

• A qualified phlebotomist visits your home, workplace, or hotel
• Blood is collected at a time convenient for you
• No clinic visit required
• Available nationwide across the UK

Both options use identical laboratory methods and clinical standards.

Understanding your Gluten Allergy Profile results

IgE-mediated gluten allergy

• Gluten IgE positive
  → Suggests true immediate-type gluten allergy

This is uncommon but may cause rapid allergic reactions.

Coeliac disease markers positive

• Tissue Transglutaminase IgA positive
• Endomysial Antibodies IgA positive
• Deamidated Gliadin IgG positive

These findings strongly suggest coeliac disease, especially when supported by symptoms and genetic risk.

Genetic susceptibility present                 

• HLA DQ2 and/or DQ8 positive

Indicates genetic predisposition to coeliac disease. This does not confirm disease, but absence of these genes makes coeliac disease extremely unlikely.

Total IgA deficiency detected

Low Total IgA may cause false-negative coeliac antibody results, making IgG-based testing essential for accurate interpretation.