Paul Bunnell (Monospot)
The Paul Bunnell (Monospot) test helps detect glandular fever caused by Epstein–Barr virus. It identifies heterophile antibodies produced during infection.
Turnaround time
1-2 days
Biomarkers count
1
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Details about Paul Bunnell (Monospot)
What is the Paul Bunnell (Monospot) test?
The Paul Bunnell, also known as the Monospot test, is a blood test used to detect heterophile antibodies produced during infection with Epstein–Barr virus (EBV), the most common cause of glandular fever.
These antibodies typically appear during the acute phase of infection and help distinguish glandular fever from other causes of prolonged fatigue, sore throat, or viral illness. The test is most useful when symptoms are present and EBV infection is suspected.
It is often requested alongside full blood count and liver function tests to support clinical assessment.
Why do I need a test for Paul Bunnell (Monospot)?
Glandular fever can present with prolonged and non-specific symptoms that overlap with other viral or bacterial infections. The Paul Bunnell test helps confirm whether EBV is the likely cause.
This test is particularly useful in adolescents and adults with persistent fatigue, sore throat, swollen glands, or unexplained illness that does not resolve as expected.
You may benefit from this test if you experience:
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Persistent fatigue or exhaustion
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Sore throat lasting more than a few days
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Swollen lymph nodes (neck or armpits)
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Fever or flu-like symptoms
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Enlarged tonsils
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Headaches or body aches
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Unexplained liver enzyme changes
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Suspected glandular fever exposure
Benefits of the Paul Bunnell (Monospot) test
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Supports diagnosis of glandular fever
Helps identify EBV-related infection. -
Detects acute immune response
Identifies heterophile antibodies produced during active infection. -
Differentiates EBV from other illnesses
Helps distinguish glandular fever from bacterial throat infections. -
Useful in prolonged fatigue assessment
Supports investigation of persistent tiredness. -
Aids clinical decision-making
Helps guide reassurance and appropriate follow-up. -
Simple blood-based investigation
Requires only a single blood sample. -
Complements other blood tests
Often interpreted alongside FBC and liver markers. -
Supports infection control awareness
Helps confirm viral cause and expected recovery course.
Step-by-step – how we offer Paul Bunnell (Monospot) testing
Clinic-based testing (UK-wide)
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Choose your nearest UK clinic
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Attend your appointment
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A qualified clinician or phlebotomist collects your blood sample
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Sample is sent to the laboratory for analysis
At-home or hotel testing (+£60)
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A qualified phlebotomist visits your home or hotel
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Blood sample collected in a comfortable, familiar setting
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No clinic visit or travel required
Both services are available UK-wide.
Your Paul Bunnell (Monospot) results explained
Negative result
A negative result means heterophile antibodies were not detected. This suggests glandular fever is unlikely at the time of testing, although early infection cannot always be excluded.
If symptoms persist, further EBV-specific antibody testing may be considered.
UK reference interpretation:
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Negative: Not detected
Positive result
A positive result indicates the presence of heterophile antibodies, supporting a diagnosis of glandular fever due to EBV infection.
This typically reflects an active or recent infection.
UK reference interpretation:
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Positive: Detected
Indeterminate / borderline result
Occasionally, results may be unclear, particularly early in infection. In such cases, additional EBV antibody testing may help clarify infection status.
UK reference interpretation:
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Borderline: Equivocal
How It Works
Simple steps to get your results
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Visit or Test at Home
Attend one of our UK or London clinics, arrange a home nurse visit, or use a finger-prick kit where available.
Get Your Results
Your sample is analysed by accredited UK laboratories, with secure results delivered directly to you.
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WhatsApp UsHow to Prepare for Your Test
Follow these guidelines for accurate results
Sample Timing
Where possible, attend your blood test between 7am and 12pm. Please wait until any short-term illness or infection has fully resolved before testing. Avoid intense exercise for 24–48 hours beforehand, as this can affect certain markers.
Fasting & Hydration
Fasting is not always required, but some tests may recommend it. If fasting is advised, avoid food for 8–12 hours before your appointment and drink water only. Stay well hydrated, as this helps with sample collection and accuracy.
Medications & Supplements
Continue prescribed medications unless advised otherwise by your clinician. Avoid vitamin, mineral, or biotin supplements for at least 24–48 hours before your test, as these can interfere with results. If you are unsure about any medication or supplement, please let us know before your appointment.
Hormones & Menstrual Cycle
For hormone-related tests, timing within your menstrual cycle may be important. If relevant, follow any specific guidance provided on your test page.
If you have any questions or special circumstances, our team is happy to advise before your appointment.