1,25-dihydroxyvitamin D (calcitriol)
This test measures 1,25-dihydroxyvitamin D, the biologically active form of vitamin D. It is used to investigate calcium imbalance, kidney disease and...
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1 day
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SelectedVitamin D Blood Test | London Blood Tests UK
Check your vitamin D levels with our £48 test. UKAS-accredited results in 1–2 days, helping identify deficiency, fatigue, bone and immune issues.
The Vitamin D Blood Test (£48) measures the level of 25-hydroxyvitamin D (25-OH D), the most accurate marker of vitamin D status. This essential test helps diagnose deficiency linked to fatigue, weakened immunity, low mood, bone pain, and muscle weakness. Results are delivered within 1–2 business days, with the option to add a GP consultation for personalised advice.
Vitamin D is a vital nutrient responsible for bone health, immune function, muscle strength, hormone balance, and overall wellbeing. Most vitamin D in the body comes from sunlight exposure, while smaller amounts are obtained from food and supplements.
The Vitamin D Blood Test measures 25-hydroxyvitamin D, the storage form of vitamin D circulating in the bloodstream. This biomarker is considered the clinical gold standard for diagnosing deficiency, insufficiency, or optimal levels.
Low vitamin D is extremely common in the UK due to limited sunlight, especially in winter. Deficiency is linked to:
Weakened immune function
Bone weakening or osteomalacia
Muscle and joint pain
Fatigue and low energy
Hormonal imbalance
Low mood or seasonal depression
At London Blood Tests, your sample is analysed in a UKAS-accredited biochemistry laboratory using high-performance immunoassay or LC-MS/MS technology for accurate results.
Vitamin D deficiency is common and often undiagnosed. Testing gives a clear picture of your levels so treatment can begin immediately.
Many non-specific symptoms — tiredness, muscle aches, low immunity — are linked to vitamin D deficiency and improve significantly with correction.
Vitamin D regulates calcium absorption and bone mineralisation. Testing is essential for preventing osteopenia, osteoporosis, and muscle weakness.
Low vitamin D is associated with more frequent colds, slower recovery, and weakened immune response. Testing supports preventive healthcare.
Vitamin D influences testosterone, oestrogen, and fertility in both men and women. Deficiency can affect menstrual cycles and reproductive function.
Knowing your exact level allows safe, tailored dosing — avoiding under-supplementing or taking excessive vitamin D.
A GP can review your results, discuss symptoms, recommend dosing, and plan follow-up testing for optimal improvement.
You may need this test if you experience:
Fatigue or low energy
Frequent infections
Muscle weakness
Bone or joint pain
Low mood, SAD, or irritability
Hair loss
Poor sleep
Slow recovery after exercise
Reduced immunity
Clinical reasons to test include:
Osteoporosis or osteopenia
Autoimmune disorders
Pregnancy or breastfeeding
Vegan/vegetarian diets
Limited sun exposure
Darker skin tones (higher deficiency risk)
Malabsorption conditions (Crohn’s, coeliac, gastric bypass)
Testing ensures your level is safe and healthy, especially before starting supplements.
Book Your Appointment
Available online or by phone.
No fasting required.
Pre-Test Review
The phlebotomist confirms whether you currently take vitamin D supplements (this helps interpretation).
Sample Collection
A simple venous blood draw takes 5 minutes.
Your sample is labelled and prepared for transport.
Laboratory Analysis
Processed at a UKAS-accredited biochemistry laboratory.
Vitamin D (25-OH) level is measured using validated immunoassays or LC-MS/MS.
Quality Control
Internal reference controls ensure accuracy.
Abnormal results are rechecked automatically.
Results Delivery
Typically available within 1–2 business days.
Access via secure online portal.
Optional GP Consultation
Discuss supplementation, dosing, treatment plan, and whether re-testing is required.
Book Your Home Visit
A phlebotomist visits your home or hotel at a time that suits you.
Professional Blood Collection
A venous blood sample is taken exactly as in the clinic.
Ideal for those preferring privacy or convenience.
Secure Same-Day Transport
The sample is packaged and couriered directly to our UKAS laboratory.
Testing & Reporting
Vitamin D (25-OH) is measured with the same clinical accuracy as in-clinic tests.
Results & Follow-Up
Results uploaded within 1–2 business days.
Optional GP consultation for personalised supplementation advice.
Vitamin D is reported as 25-hydroxyvitamin D (nmol/L).
Deficient: < 30 nmol/L
Insufficient: 30–50 nmol/L
Adequate: 50–75 nmol/L
Optimal: 75–150 nmol/L
High: > 150 nmol/L
Potential toxicity: > 250 nmol/L
Low levels can indicate:
Limited sun exposure
Dietary deficiency
Malabsorption
Increased demand (pregnancy, breastfeeding)
Chronic illness
Obesity or metabolic issues
High levels typically occur due to excessive supplementation. This requires monitoring to avoid calcium imbalance or kidney strain.
A GP can interpret your result and provide safe supplementation guidance.
Can I book this test if I’m outside London?
Yes. Our home finger-prick kits are available nationwide, allowing you to access testing and GP consultations remotely.
Simple steps to get your results
Choose your test online and book in seconds. Select your preferred clinic location or home visit option.
Attend one of our UK or London clinics, arrange a home nurse visit, or use a finger-prick kit where available.
Your sample is analysed by accredited UK laboratories, with secure results delivered directly to you.
Take control of your health with London Blood Tests
WhatsApp UsFollow these guidelines for accurate results
Where possible, attend your blood test between 7am and 12pm. Please wait until any short-term illness or infection has fully resolved before testing. Avoid intense exercise for 24–48 hours beforehand, as this can affect certain markers.
Fasting is not always required, but some tests may recommend it. If fasting is advised, avoid food for 8–12 hours before your appointment and drink water only. Stay well hydrated, as this helps with sample collection and accuracy.
Continue prescribed medications unless advised otherwise by your clinician. Avoid vitamin, mineral, or biotin supplements for at least 24–48 hours before your test, as these can interfere with results. If you are unsure about any medication or supplement, please let us know before your appointment.
For hormone-related tests, timing within your menstrual cycle may be important. If relevant, follow any specific guidance provided on your test page.
If you have any questions or special circumstances, our team is happy to advise before your appointment.
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